Facilities

hood2 All of our facilities are state of the art and adequate for the overall functions of the Center and to implement the goals of the contract proposal.  We provide a brief description below.

Chemistry

We have state of the art chemistry facilities with fourteen 9 ft hoods, all are plumbed with Nitrogen, gas and vacuum available for use with the center.  We can perform low and high temperature, low and high pressure, and non-oxygen atmosphere reactions.  We have expertise in natural product synthesis, analog chemistry development, asymmetric syntheses for mg to 100 gram reactions.

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Analytical

We have a full dedicated Varian 400 mHz multinuclear (C, H, N, P, B) NMR installed in December of 2006.  This is fully integrated for WinNMR for offline workup of spectra and data analysis.   We have full gram to kilogram separation and purification using Biotage instruments. nmr1

Our dedicated analytical facilities contain a Shimadzu GC/MS, LC/MS.   The facility is equipped with technologies for the fractionation of complex protein mixtures coupled with mass spectrometry as well as metabolite profiling from urine, plasma and/or serum samples. In addition the GUMC proteomics and metabolomics core facility is equipped with a 4800 MALDI-TOF-TOF Mass Spectrometer (Applied Biosystems), QSTAR Elite Hybrid LCMS/MS system, a nanoTEMPO MDLC system online with a Probot MALDI spotter (LC packings) for proteomics based experiments. In addition, the core has a high resolution UPLC system coupled to the QTOF Premiere mass spectrometer (Waters Corporation) for accurate mass determination which is used for the identification of metabolite biomarkers from biofluids. The core also has a 4000 Q Trap (Applied Biosystems) for validation and quantitation studies for metabolite biomarkers as well as for post translational modification hplc determination of proteins. These instruments can provide a range of techniques to analyze different aspects of a fractionated sample.  Current equipment inventory include the following: chemical fume hood, 4800 MALDI TOF-TOF, micro-pipets with yearly calibration, Nano HPLC, QSTAR Elite Hybrid LCMS/MS System, TEMPO MDLC system, DIGE Imager and Spot Picker, QTOF Premier system, Nano Acquity UPLC systems (x2), Acquity UPLC system (x2), and 4000 Q Trap system.

Tissue Culture biotage1

Our tissue culture facility is a stand-alone room with Biohazard Level-2 capacity.  The facility is equipped with cell culture CO2 chambers, laminar flow hoods with vacuum and N2, -80 upright freezer and walk-in freezer room.

Preclinical

We have capacity for a full range of xenograft, ADME, dose ranging and toxicology studies.  The Animal Research Resource, under the direction of a full-time faculty member and staffed by three full-time research technicians, is located in space rented from the AAALAC accredited Georgetown University Division of Comparative Medicine (DCM: Full  tissueculture Accreditation; Animal Welfare Assurance Number A3282-01). The DCM also is an USDA approved facility. The Animal Research Resource is conveniently accessed from all scientific space through interconnecting corridors. Immune-deficient animals are housed in barrier facilities and Biosafety Level-2 areas are also available. The equipment in the immune-deficient housing areas includes state-of-the-art laminar flow colony racks and workstations. All related supplies for manipulations (e.g., drug administrations, cell line inoculations) are located in the anteroom to the colony. The Animal Facility is equipped with Biohazard Level-2 housing for carcinogen and chemotherapeutic experiments, and an additional room for manipulations and necropsy. This resource allows access to specialized areas for surgical procedures (chronic and acute surgical suites), preparation of materials for animal care and husbandry, diagnostic tools (Kodak Ektachem chemistry analyzer, Hematology analyzer, stereoscope), necropsy and radiology. The shared resource also provides access to surgical instruments (forceps, scalpels, scissors, microsurgical instruments), anesthetic supplies, and technical services. The DCM employs two full-time veterinarians available for consultation. All work is carried out under protocols approved by the Georgetown University Animal Care and Use Committee (GUACUC).

Unique to our center is our ability to rapidly access in vitro and in vivo ADME and design and synthesize molecules to address ADME deficiencies.  Given our robust animal models group, we routine develop chemistry for successful scale-up synthesis of desired chemical compounds in the 1-50 gram scale.    We have a track record of participating with diverse groups including government, industry, academic and research institutes towards the discovery of new drugs.